9J0A
Complex structure of ANKRD11/STAG2/RAD21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 280.535, 48.773, 290.906 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.880 - 3.300 |
R-factor | 0.2351 |
Rwork | 0.233 |
R-free | 0.27920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qnx |
RMSD bond length | 0.003 |
RMSD bond angle | 0.627 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.196 | 1.406 |
Rmeas | 0.214 | 1.523 |
Rpim | 0.084 | 0.580 |
Number of reflections | 60932 | 2973 |
<I/σ(I)> | 8 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.45 | 6.76 |
CC(1/2) | 0.997 | 0.606 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 289 | 0.02 M citric acid, 0.08 M BIS-TRIS propane (pH 8.8), and 16% w/v polyethylene glycol 3,350 |