9IY2
Immune complex of HEV-E2s, nAb 8C11 and nAb 8H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.465, 248.941, 76.841 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 36.651 - 3.476 |
| R-factor | 0.2256 |
| Rwork | 0.223 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rkd 4m61 2DTG |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.601 |
| High resolution limit [Å] | 3.476 | 3.476 |
| Rmeas | 0.237 | |
| Rpim | 0.124 | |
| Number of reflections | 32075 | 2967 |
| <I/σ(I)> | 6.81 | |
| Completeness [%] | 93.5 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 0.2M MgSO4 + 8% glycerinum + 11% PEG 40,000 |
