9IY0

anti-HEV mAb 8H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	37.089, 103.062, 52.044
Unit cell angles	90.00, 90.53, 90.00

Refinement procedure

Resolution	25.970 - 1.970
R-factor	0.1939
Rwork	0.192
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fns
RMSD bond length	0.004
RMSD bond angle	0.649
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.970	5.350	1.970
Rmerge		0.028	0.542
Rmeas		0.033	0.633
Rpim		0.017	0.325
Number of reflections	27054	1397	1304
<I/σ(I)>	10.1
Completeness [%]	98.5	99.2	94.6
Redundancy	3.8	3.8	3.6
CC(1/2)		0.998	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	2M Mg(Ac)2 and 18% PEG 3350