Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.400, 164.933, 56.588 |
| Unit cell angles | 90.00, 111.84, 90.00 |
Refinement procedure
| Resolution | 44.302 - 2.000 |
| Rwork | 0.188 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.302 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.144 | 1.085 |
| Number of reflections | 50921 | 3701 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 98.0 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HM(PEGs-E9)-D6 (0.18M NH4Cl, 24% PEG 3350) |
