9ISA

Dimeric amylosucrase from Deinococcus geothermalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979330
Spacegroup name	P 41 21 2
Unit cell lengths	149.917, 149.917, 188.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.220 - 2.690
R-factor	0.17855
Rwork	0.176
R-free	0.21985
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uer
RMSD bond length	0.007
RMSD bond angle	1.711
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.840
High resolution limit [Å]	2.690	2.690
Rmerge	0.099	0.458
Number of reflections	49477	7656
<I/σ(I)>	9.8
Completeness [%]	87.7
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	0.1 M HEPES sodium pH 7.5, 10% v/v 2-Propanol, 20% w/v Polyethylene glycol 4,000