9IRM
Structure of ClpP from Staphylococcus aureus in complex with ZG283
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.410, 127.080, 146.100 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 31.890 - 1.810 |
| R-factor | 0.21228 |
| Rwork | 0.212 |
| R-free | 0.22614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | autoPROC |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.890 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 314521 | 23142 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 97.6 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.998 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 35% 2-Methyl-2,4-pentanediol, 0.1 M HEPES/Sodium hydroxide, pH 7.5, 0.2 M Sodium chloride |
