9IQM

CatM-L6S, a SnoaL-like protein in complex with substrate mimic L6S

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	C 2 2 21
Unit cell lengths	38.617, 99.719, 58.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.010 - 1.700
R-factor	0.2089
Rwork	0.208
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9iq5
RMSD bond length	0.009
RMSD bond angle	1.143
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.010	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	12789	1178
<I/σ(I)>	13.37	2.38
Completeness [%]	99.0
Redundancy	10.2
CC(1/2)	0.997	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	2.0 M Ammonium sulfate, 0.1 M Sodium HEPES 7.5, 2 % v/v PEG 400