9IQD
Apo-MicM,homologous of AkaM, SnoaL-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.306, 72.732, 65.403 |
| Unit cell angles | 90.00, 112.21, 90.00 |
Refinement procedure
| Resolution | 59.540 - 1.770 |
| R-factor | 0.1832 |
| Rwork | 0.181 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9iq7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.540 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 53998 | 5331 |
| <I/σ(I)> | 14.13 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.997 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291.15 | 20 % w/v Polyethylene glycol 6,000, 100 mM tri-Sodium citrate; pH 5.0 |
