9IQ7

SacM-homologous of AkaM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	P 43 21 2
Unit cell lengths	52.182, 52.182, 202.928
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.730 - 1.770
R-factor	0.204
Rwork	0.203
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.929
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.730	1.830
High resolution limit [Å]	1.770	1.770
Number of reflections	27227	2760
<I/σ(I)>	26.03
Completeness [%]	95.5
Redundancy	15.1
CC(1/2)	0.995	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	2.0 M Ammonium sulfate 0.1 M Sodium HEPES 7.5 2 % v/v PEG 400