9IOV
Gossypol bound lactate dehydrogenase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.369, 110.924, 217.546 |
| Unit cell angles | 89.98, 90.04, 88.37 |
Refinement procedure
| Resolution | 35.560 - 3.980 |
| R-factor | 0.2401 |
| Rwork | 0.238 |
| R-free | 0.27315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.630 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.560 | 4.170 |
| High resolution limit [Å] | 3.980 | 4.070 |
| Rmerge | 0.051 | 0.051 |
| Number of reflections | 63704 | 63704 |
| <I/σ(I)> | 9.3 | 9.3 |
| Completeness [%] | 97.0 | |
| Redundancy | 2.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 8% Tacsimate (pH 7.0), 20% PEG 3350 |
