9IMB

The crystal structure of human m5C methyltransferase NSUN6 bound to its S-adenosyl-L-methionine analog and a NECTIN-2 3'-UTR RNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97852
Spacegroup name	P 1
Unit cell lengths	61.814, 72.441, 77.150
Unit cell angles	62.48, 83.38, 69.62

Refinement procedure

Resolution	43.000 - 2.900
R-factor	0.2101
Rwork	0.207
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wwq
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.003	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.124	0.483
Number of reflections	24167	2643
<I/σ(I)>	5.7	1.7
Completeness [%]	98.2	97.74
Redundancy	3.5
CC(1/2)	0.988	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20% PEG 3350, 1% w/v tryptone, 0.001 M sodium azide, 0.05 M HEPES sodium (pH 7.0)