9ILS
The GmvT toxin in complex with the C-terminal fragment of its antitoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.572, 78.572, 131.937 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.680 - 2.750 |
| R-factor | 0.2501 |
| Rwork | 0.249 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ak8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.162 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.132 | 1.410 |
| Number of reflections | 6761 | 658 |
| <I/σ(I)> | 43.5 | 3 |
| Completeness [%] | 100.0 | |
| Redundancy | 36.5 | |
| CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.4-1.9 M KH2PO4 and 0.1 M HEPES pH 7.0. |
