9ILB

HUMAN INTERLEUKIN-1 BETA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1996-04-01
Detector	RIGAKU
Spacegroup name	P 43
Unit cell lengths	55.020, 55.020, 77.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.280
Rwork	0.157
R-free	0.21000
Structure solution method	OTHER
Starting model (for MR)	2i1b
RMSD bond length	0.013
RMSD bond angle	2.130
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.370
High resolution limit [Å]	2.280	2.280
Rmerge	0.056	0.280
Number of reflections	10313
Completeness [%]	100.0	86
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.4 (M)
2	1	reservoir	dithiothreitol	1 (mM)
3	1	reservoir	HEPES	100 (mM)