9IIT
Full length structure of FKP in complex with GTP and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.821, 96.061, 127.369 |
| Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
| Resolution | 29.150 - 2.300 |
| R-factor | 0.2005 |
| Rwork | 0.200 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 6LH3 and 6LH5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.550 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.077 | 0.940 |
| Number of reflections | 111983 | 11074 |
| <I/σ(I)> | 22.8 | 1.6 |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 0.918 | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 8% Tascimate, 20% PEG 3350, 15mM GTP, 15mM MgCl2 |
