9IIE
Cryogenic Temperature Crystal Structure of Fc Fragment of Human IgG1 from Biosimilar VEGF-Trap
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-03 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.920, 80.480, 137.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.840 - 3.140 |
| R-factor | 0.2621 |
| Rwork | 0.257 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.503 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.080 | 3.220 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Number of reflections | 9844 | 683 |
| <I/σ(I)> | 2.26 | |
| Completeness [%] | 96.5 | 98.6 |
| Redundancy | 3.6 | 3.9 |
| CC(1/2) | 0.748 | 0.155 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Bis-Tris pH 6.5, 25% w/v Polyethylene glycol 3350 |
