9IBF

Crystal structure of hFcgammaRI-FAb complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-16
Detector	DECTRIS EIGER2 X CdTe 9M
Wavelength(s)	0.61992
Spacegroup name	P 21 21 21
Unit cell lengths	121.715, 126.491, 141.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.700 - 3.200
Rwork	0.259
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.775
Data reduction software	xia2
Data scaling software	Aimless (1.12.14)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.806	3.340
High resolution limit [Å]	3.200	3.200
Rmerge	0.946	4.170
Rpim	0.192	0.855
Number of reflections	36765	4412
<I/σ(I)>	4.1	1.2
Completeness [%]	100.0	100
Redundancy	25.1	25.4
CC(1/2)	0.975	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	MOPS/HEPES buffer pH 7.5 0.12 M alcohol mixture 30% PEG20k-PEG MME550 mixture