9I9U

CARM1 in complex with arg-sDMA analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	74.910, 98.392, 208.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.880 - 2.200
R-factor	0.212
Rwork	0.210
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.793
Data reduction software	XDS (2.3.15)
Data scaling software	Aimless (0.7.8)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.880	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.139	2.412
Rmeas	0.145	2.501
Rpim	0.039	0.658
Number of reflections	78119	4387
<I/σ(I)>	14.1	1.4
Completeness [%]	99.0	98.3
Redundancy	13.8	14.2
CC(1/2)	0.999	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	12% PEG6000, 100 mM hepes pH7