9I5R

Interactions between Pex3 and Pex19 peptide in yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97624
Spacegroup name	C 2 2 21
Unit cell lengths	68.579, 137.224, 111.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.612 - 2.620
Rwork	0.209
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.631
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	111.530	111.530	2.740
High resolution limit [Å]	2.620	9.080	2.620
Rmerge	0.101	0.031	4.759
Rmeas	0.111	0.034	5.184
Rpim	0.045	0.014	2.048
Number of reflections	16218	449	1961
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	11.4	9.5	12.2
CC(1/2)	0.999	0.999	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	ammonium phosphate 0.2M + PEG 3350 20%, ph 7.0