9I5B
Crystal structure of perlecan region 3 mutant (P1019L) construct I876-V1272 including one laminin IV-like and four laminin EGF-like domains.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.650, 74.968, 81.368 |
| Unit cell angles | 75.23, 73.12, 80.11 |
Refinement procedure
| Resolution | 47.670 - 2.520 |
| Rwork | 0.188 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.870 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 2.580 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Number of reflections | 41874 | 3056 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.994 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295.15 | 0.85M succinic acid, pH 7 |
