9HYQ

BT984 a GH139 rhamnogalacturonan II exo-a-1,2-(2-Omethyl)-fucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	101.201, 138.322, 195.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.478 - 2.700
Rwork	0.218
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.478	2.760
High resolution limit [Å]	2.700	2.700
Number of reflections	75053	4450
<I/σ(I)>	10.4	1.3
Completeness [%]	98.9
Redundancy	3.3
CC(1/2)	0.997	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20 % PEG 3000 0.1 M Tris pH8.5 10 mg/ml