9HU9

Glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system. Complex with UDP-GlcNAc.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	76.639, 121.392, 174.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.700 - 2.400
Rwork	0.189
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.469
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.700	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.099	0.902
Number of reflections	64276	6327
<I/σ(I)>	5.62	0.58
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	0.990	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	289	ammonium hydrogen citrate, PEG 3350