9HIZ
Complex of the Nanofitin Sac7d-C3(C24A) with a human IgG1 Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97620 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.270, 136.840, 69.760 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 69.760 - 2.900 |
| R-factor | 0.22991 |
| Rwork | 0.227 |
| R-free | 0.27928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.443 |
| Data reduction software | XDS (Jun 30, 2023) |
| Data scaling software | XDS (Jun 30, 2023) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 136.800 | 136.800 | 3.000 |
| High resolution limit [Å] | 2.900 | 30.000 | 2.900 |
| Rmerge | 0.099 | 0.068 | 0.748 |
| Rmeas | 0.119 | 0.085 | 0.902 |
| Number of reflections | 27577 | 26 | 2708 |
| <I/σ(I)> | 7.74 | ||
| Completeness [%] | 97.0 | ||
| Redundancy | 3.1 | ||
| CC(1/2) | 0.994 | 0.993 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 24 % (w/v) PEG8000, 0.1 M Tris/HCl pH 8.5 |
