9H6B

Crystal structure of the E. coli F-plasmid VapBC toxin-antitoxin complex (VapB T3N, A13P, L16R)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979261
Spacegroup name	P 32 2 1
Unit cell lengths	89.590, 89.590, 115.978
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.450 - 2.800
R-factor	0.2346
Rwork	0.230
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.907
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.500	46.500	2.870
High resolution limit [Å]	2.800	12.530	2.800
Rmerge	0.093	0.036	2.806
Rmeas	0.098	0.038	2.956
Number of reflections	13688	188	993
<I/σ(I)>	14.13
Completeness [%]	99.9
Redundancy	10.1
CC(1/2)	0.999	0.998	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2 M Lithium sulphate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 26 % PEG200