9H4S

Structure of fertilization-blocking monoclonal antibody IE-3 VHVL bound to the ZP-N1 domain of mouse ZP2 (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976251
Spacegroup name	P 43
Unit cell lengths	121.750, 121.750, 71.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.310 - 2.020
R-factor	0.1897
Rwork	0.189
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Partially refined model of PDB ID 6GF4
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.314	2.090
High resolution limit [Å]	2.020	2.020
Rmeas	0.056	1.478
Rpim	0.021	0.553
Number of reflections	68723	48401
<I/σ(I)>	24.5	1.4
Completeness [%]	100.0	100
Redundancy	6.8	7.1
CC(1/2)	1.000	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	2.0 M lithium sulfate, 0.2 M ammonium sulfate, 0.1 tri-sodium citrate pH 5.6