9H4R

Structure of fertilization-blocking monoclonal antibody IE-3 VHVL bound to the ZP-N1 domain of mouse ZP2 (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976251
Spacegroup name	P 21 21 21
Unit cell lengths	91.640, 92.290, 91.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.160 - 1.530
R-factor	0.2095
Rwork	0.206
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P43-Q138 of PDB ID 5II6; chain H of PDB ID 4AMK; chain L of PDB ID 3MBX
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.164	1.580
High resolution limit [Å]	1.530	1.530
Rmeas	0.030	1.376
Rpim	0.016	0.721
Number of reflections	115130	11474
<I/σ(I)>	18.6	1
Completeness [%]	98.5	99.2
Redundancy	3.3	3.4
CC(1/2)	1.000	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	24% (v/v) PEG 3350, 0.2 M sodium tartrate, 0.1 M Na-HEPES pH 6.5