9H0M

Structure of avian Pit54 SRCR 1-2 in complex with hemoglobin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 2
Unit cell lengths	101.534, 157.592, 175.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.680 - 2.640
R-factor	0.2026
Rwork	0.201
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.839
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.680	2.840
High resolution limit [Å]	2.640	2.640
Rmeas	0.117	1.345
Number of reflections	55820	2792
<I/σ(I)>	11.92	1.55
Completeness [%]	95.5
Redundancy	5.62
CC(1/2)	0.998	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	281	0.3 M Ammonium tartrate 20% PEG3350