9GWT

crystal structure of 23ME-00610 Fab in complex with human CD200R1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-07
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	.999
Spacegroup name	P 41 21 2
Unit cell lengths	98.654, 98.654, 279.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.030 - 2.890
R-factor	0.2036
Rwork	0.202
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.031	3.077
High resolution limit [Å]	2.887	2.887
Number of reflections	27539	1377
<I/σ(I)>	16.3
Completeness [%]	94.3
Redundancy	25.1
CC(1/2)	0.998	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Sodium Cacodylate 0.1M, Glycerol 10% (v/v), NaCl 1M, PEG 600 30.0% (w/v)