9GW5
type-I interferon autoantibodies pmab3, pmab19 and pmab14 in complex with Interferon alpha-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953721 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 166.216, 166.216, 190.135 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 4.000 |
| R-factor | 0.2958 |
| Rwork | 0.294 |
| R-free | 0.32360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 4.128 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.781 | 8.869 |
| Rmeas | 0.786 | 8.923 |
| Rpim | 0.128 | 1.379 |
| Number of reflections | 13477 | 1038 |
| <I/σ(I)> | 8.8 | 1.1 |
| Completeness [%] | 98.7 | 86.9 |
| Redundancy | 79 | 81.5 |
| CC(1/2) | 0.996 | 0.686 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M Hepes pH 7.5 1.9 (NH4)2SO4 |
