9GVL

type-I interferons autoantibody pmab15 in complex with Interferon alpha-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-28
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.953721
Spacegroup name	P 1 21 1
Unit cell lengths	66.159, 83.719, 70.264
Unit cell angles	90.00, 113.80, 90.00

Refinement procedure

Resolution	14.970 - 2.006
R-factor	0.217
Rwork	0.214
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	XDS (Jun 30, 2023 (BUILT 20230630))
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.000	2.041
High resolution limit [Å]	2.000	2.006
Rmerge	0.202	2.611
Rmeas	0.225	2.902
Rpim	0.098	1.667
Number of reflections	46986	2310
<I/σ(I)>	3.6	0.6
Completeness [%]	100.0	99.7
Redundancy	5	5.1
CC(1/2)	0.991	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	0.2 M (NH4)2SO4 0.1 M NaAcetate pH 4.6 30 %w/w PEG 2000 MME