9GV4
TBA G-quadruplex binding nanobody (free form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 24.125, 84.257, 97.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.721 - 1.534 |
| R-factor | 0.1732 |
| Rwork | 0.170 |
| R-free | 0.20280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.941 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.721 | 1.570 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.068 | 0.441 |
| Rmeas | 0.074 | 0.480 |
| Rpim | 0.029 | 0.188 |
| Total number of observations | 7056 | |
| Number of reflections | 15415 | 1099 |
| <I/σ(I)> | 16.1 | 3.2 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.999 | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 298 | 1.6 M Ammonium sulphate 0.1 M HEPES, pH=7.25 cryoprotection=25% glycerol |
