9GSQ

DNA binding domain of J-DNA Binding Protein 3 (JBP3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.966
Spacegroup name	C 1 2 1
Unit cell lengths	112.701, 40.937, 100.595
Unit cell angles	90.00, 123.82, 90.00

Refinement procedure

Resolution	83.576 - 1.700
Rwork	0.174
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.164
Data reduction software	DIALS
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.576	83.580	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmeas	0.050	0.024	0.630
Number of reflections	41694	304	2186
<I/σ(I)>	11.1	28.8	0.75
Completeness [%]	99.0	93
Redundancy	2.9	3	2.9
CC(1/2)			0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.2 M potassium sodium tartrate tetrahydrate, 20% w/v polyethylane glycol 3,350