9GOY
Crystal structure of Fab E2-RecA in complex with CD38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-09-18 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.290, 61.070, 124.120 |
| Unit cell angles | 90.00, 108.60, 90.00 |
Refinement procedure
| Resolution | 47.220 - 2.700 |
| R-factor | 0.24481 |
| Rwork | 0.243 |
| R-free | 0.27926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.220 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.307 | 3.320 |
| Number of reflections | 24595 | 1821 |
| <I/σ(I)> | 6.62 | 0.72 |
| Completeness [%] | 99.8 | 99.64 |
| Redundancy | 7 | |
| CC(1/2) | 0.990 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 70% v/v MPD, 0.1M HEPES, pH 7.5 |
