9GOX
Crystal structure of Fab B6-D9 in complex with CD38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-05-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 166.045, 166.045, 78.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.270 - 1.840 |
| R-factor | 0.17616 |
| Rwork | 0.174 |
| R-free | 0.20831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.426 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.270 | 2.020 |
| High resolution limit [Å] | 1.840 | 1.843 |
| Rmerge | 0.136 | |
| Rmeas | 0.143 | |
| Number of reflections | 73151 | 1843 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 77.0 | 68.8 |
| Redundancy | 11.4 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 7 % v/v T-mate pH 7.0, 0.1 M HEPES pH 7.2, 20 % v/v PEG Smear Medium (BCS screen, Molecular Dimensions) |
