9GNN

Structure of SENP5 in complex with SUMO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	66.162, 87.902, 116.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.610 - 2.360
R-factor	0.2165
Rwork	0.213
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold
Data reduction software	autoPROC
Data scaling software	AutoProcess
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.510
High resolution limit [Å]	2.360	2.360
Number of reflections	23655	1183
<I/σ(I)>	10.8
Completeness [%]	90.1
Redundancy	6.5
CC(1/2)	0.997	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Sodium chloride, Phosphate/citrate 4.2, 20 % w/v PEG 8000