9GMU
Structure ofhuman aM ligand binding domain in complex with the aCR3 nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.564, 56.142, 137.444 |
| Unit cell angles | 90.00, 128.61, 90.00 |
Refinement procedure
| Resolution | 49.250 - 3.800 |
| R-factor | 0.2453 |
| Rwork | 0.242 |
| R-free | 0.29760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.832 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.250 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 11989 | 1194 |
| <I/σ(I)> | 7.48 | 1.9 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.997 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 100 mM HEPES, pH 7.0, 1.5 M Li2S04. |
