9GJQ
Crystal structure of humanised cereblon from Magnetospirillum gryphiswaldense bound by dihydrouracil-indole compound [1-(1H-indol-6-yl)dihydro-2,4(1H,3H)-pyrimidinedione].
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95380 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.782, 82.305, 52.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.830 - 1.393 |
| R-factor | 0.2004 |
| Rwork | 0.200 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.070 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.829 | 1.467 |
| High resolution limit [Å] | 1.393 | 1.393 |
| Number of reflections | 18772 | 939 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 94.2 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.999 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 % PEG8000, 8 % ethylene glycol and 100 mM Na-HEPES buffer (pH 7.5) |
