9GJ0

Eugenol Oxidase (EUGO) from Rhodococcus jostii RHA1, mutant B1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.969
Spacegroup name	P 32 2 1
Unit cell lengths	109.770, 109.770, 244.985
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	95.060 - 2.760
R-factor	0.20898
Rwork	0.206
R-free	0.26558
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.426
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	244.990	2.860
High resolution limit [Å]	2.760	2.760
Rmerge	0.128	3.475
Rmeas	0.131	3.576
Rpim	0.030	0.839
Total number of observations	836685	83399
Number of reflections	43581	4650
<I/σ(I)>	18.4	0.9
Completeness [%]	97.0
Redundancy	19.2	17.9
CC(1/2)	0.999	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Ammonium Sulphate, 20% PEG3350