9GIO
Crystal structure of the VHL-EloC-EloB complex with a covalent compound bound to C77 of VHL.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.781, 59.312, 97.316 |
| Unit cell angles | 90.00, 100.12, 90.00 |
Refinement procedure
| Resolution | 29.660 - 1.486 |
| R-factor | 0.2054 |
| Rwork | 0.204 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 1.622 |
| High resolution limit [Å] | 1.486 | 1.486 |
| Number of reflections | 47660 | 2383 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 92.1 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.987 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12 % PEG8000 and 100 mM MES buffer (pH 6.5). |
