9GFJ
Crystal structure of ASO binding Fab fragment with ASO143
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.539, 57.694, 63.046 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 40.990 - 1.480 |
| R-factor | 0.1842 |
| Rwork | 0.183 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.990 | 1.580 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.059 | 0.640 |
| Number of reflections | 76055 | 13462 |
| <I/σ(I)> | 8.57 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.46 | |
| CC(1/2) | 0.999 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.0, 20% PEG8000 |
