9GDK

Jumonji domain-containing protein 1C with crystallization epitope mutations L2440Y:G2444H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	45.200, 98.250, 87.400
Unit cell angles	90.00, 92.04, 90.00

Refinement procedure

Resolution	31.369 - 1.780
Rwork	0.209
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.859
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.77))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.370	1.830
High resolution limit [Å]	1.780	1.780
Rmerge	0.086	0.936
Number of reflections	71577	18899
<I/σ(I)>	12	1.7
Completeness [%]	98.1	99.4
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	LFS well F04 20% PEG3350 10% ethylene glycol 0.1M bis-tris-propane pH 6.5 0.2M potassium thiocyanate