9G97
Lipid III flippase WzxE with NB10 nanobody in outward-facing conformation at 0.9688 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9688 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 270.550, 38.470, 56.830 |
| Unit cell angles | 90.00, 93.50, 90.00 |
Refinement procedure
| Resolution | 51.190 - 2.310 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.020 | 2.350 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 25893 | 1276 |
| <I/σ(I)> | 7.8 | 1.1 |
| Completeness [%] | 98.7 | 98.2 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.43 | 293 | sodium acetate, PEG400, lithium sulfate, zinc chloride, E. coli polar lipids, monoolein |
