Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 61 |
| Unit cell lengths | 133.306, 133.306, 58.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.630 - 1.650 |
| Rwork | 0.154 |
| R-free | 0.17850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.808 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.630 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.033 | 0.335 |
| Number of reflections | 70220 | 3349 |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 98.5 | 95.4 |
| Redundancy | 20.7 | |
| CC(1/2) | 0.999 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | Structure Screen condition E9 Ethylene glycol as cryoprotectant |
