9G7K
Staphylococcus aureus MazF in complex with Nanobody 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.190, 61.270, 97.310 |
| Unit cell angles | 89.85, 83.89, 67.19 |
Refinement procedure
| Resolution | 47.863 - 2.087 |
| R-factor | 0.191407936985 |
| Rwork | 0.189 |
| R-free | 0.23353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.528 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.863 | 2.210 |
| High resolution limit [Å] | 2.087 | 2.087 |
| Rmerge | 0.054 | 0.355 |
| Number of reflections | 61618 | 9022 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 93.8 | 85 |
| Redundancy | 1.96 | 1.91 |
| CC(1/2) | 0.997 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 0.2 M ammonium sulphate, 0.1 M Na-cacodylate pH 4.6, 30% (w/v) PEG-4000 |
