9G5Z

Nanobody-mediated activation and inhibition of Staphylococcus aureus MazF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 31 2 1
Unit cell lengths	102.100, 102.100, 101.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.575 - 3.429
R-factor	0.24628427461
Rwork	0.244
R-free	0.28311
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.575	3.640
High resolution limit [Å]	3.429	3.429
Number of reflections	8391	1237
<I/σ(I)>	8.14
Completeness [%]	98.6
Redundancy	9.9
CC(1/2)	0.995	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.01 M Cobalt(II) chloride hexahydrate, 0.1 M MES monohydrate pH 6.5, 1.8 M Ammonium sulfate, 20% glycerol