9G4G
Staphylococcus aureus MazF in complex with Nanobody 9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.090, 133.750, 100.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.084 - 2.896 |
R-factor | 0.255352559346 |
Rwork | 0.253 |
R-free | 0.30320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.571 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.084 | 3.070 |
High resolution limit [Å] | 2.896 | 2.896 |
Number of reflections | 7201 | 1195 |
<I/σ(I)> | 9.51 | 2.65 |
Completeness [%] | 93.5 | |
Redundancy | 4.8 | |
CC(1/2) | 0.990 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M ammoniumsulphate, 0.1 M Na-cacodylate pH 6.5, 30% (w/v) PEG-8000 |