9G2A

Staphylococcus aureus MazF in complex with nanobody 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 1
Unit cell lengths	37.970, 51.650, 70.340
Unit cell angles	81.26, 81.61, 71.65

Refinement procedure

Resolution	37.859 - 2.047
R-factor	0.242874958843
Rwork	0.240
R-free	0.30665
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.098
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.859	2.170
High resolution limit [Å]	2.047	2.047
Number of reflections	29592	4611
<I/σ(I)>	8.75
Completeness [%]	94.0
Redundancy	1.96
CC(1/2)	0.997	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% w/v PEG20000, 20% w/v PEG-MME 550, 0.1 M MOPS/HEPES pH 7.5