9G11

sugar/H+ symporter STP6 in inward occluded conformation with glucose bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96863
Spacegroup name	P 32 2 1
Unit cell lengths	122.221, 122.221, 139.091
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.230 - 3.200
R-factor	0.2303
Rwork	0.227
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.230	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.226	1.168
Rmeas	0.258	1.330
Rpim	0.123	0.627
Total number of observations	80631	15369
Number of reflections	19456	3591
<I/σ(I)>	7.3	1.5
Completeness [%]	96.5
Redundancy	4.1	4.3
CC(1/2)	0.981	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292.15	30% PEG smear (PEG2000, PEG3000, PEG3500, PEG4000, PEG5000), 0.1M Hepes pH 8.0, 0.4M NaCl