9FZC
Structure of OmpA-short in complex with nanobody Nb01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.048, 55.296, 107.805 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 107.790 - 2.270 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.594 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.790 | 2.314 |
| High resolution limit [Å] | 2.270 | 2.275 |
| Rmerge | 0.146 | 0.876 |
| Number of reflections | 30638 | 1534 |
| <I/σ(I)> | 10.7 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.76 | |
| CC(1/2) | 0.997 | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M sodium acetate pH 5.5, 0.2 M calcium acetate, 25 % PEG MME 2K |
