9FZ4

SARS CoV-2 nsp10 in complex with theExoN domain from nsp14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	62.034, 70.724, 109.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.940 - 2.440
R-factor	0.1863
Rwork	0.181
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.182
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	2.480
High resolution limit [Å]	2.440	2.440
Rmerge	0.200	0.123
Number of reflections	18581	915
<I/σ(I)>	12.1
Completeness [%]	100.0	100
Redundancy	13
CC(1/2)	0.994	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.04-0.24 M Amino acids, 0.1 M buffer system 2 (pH 7.5), 16-38% v/v precipitant mix 2.