9FVK

Crystal structure of amyloidogenic light chain AL-55 in open conformation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 2 2 21
Unit cell lengths	111.817, 208.381, 72.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.640 - 2.640
R-factor	0.1954
Rwork	0.194
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.573
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.640	2.730
High resolution limit [Å]	2.640	2.640
Rmeas	0.363
Number of reflections	25185	2478
<I/σ(I)>	6.27
Completeness [%]	99.9
Redundancy	13.8
CC(1/2)	0.987	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M MIB (Sodium malonate dibasic monohydrate, Imidazole, Boric acid) pH 5.0, and 25 % w/v PEG 1500